Importing the First Image Volume

The first image volume has the following special properties:

No other images can be displayed until the first image volume has been loaded or imported
All subsequent image volumes must have the same dimensions as the first image volume and will be discarded if they do not
Loading or importing a new first image volume will cause all other images to be discarded if they do not match the dimensions of the newly loaded or imported first image volume
Only the first image volume can be modified or saved

Files in the Analyze and MINC Image formats must be loaded rather than imported.

Imported images must be 8- or 16-bit grayscale images in one of the following formats:

uncompressed Tagged Image File Format (TIFF)
zipped TIFF format
Graphics Interchange Format (GIF)
Joint Photographic Experts Group (JPEG) format
Bitmap format (BMP)
Flexible Images Transport System (FITS) format

To import the first volume image:

From the main MultiTracer window "File" menu, choose "Import First Volume ..."
Select the file that you want to open from the "Open" dialog box. If the file is not in the current directory, the popup menu in the upper left of the dialogue box will let you move up the directory tree, or you can double click on a subdirectory icon to move down the directory tree.
Once the file is selected, click the "Open" button in the dialogue box to load the file.
Large files may take several seconds to load
Once the file is loaded, the image should appear in the display region. If no prior image has been loaded, MultiTracer will automatically take you to a plane in the middle of the image and will adjust the intensity of the display to a (usually) reasonable value. If the display appears black, this may reflect a problem with the automatic intensity adjustment, and you may need to set the intensity manually.

If you have already loaded or imported a file and choose to import a new first image volume with different dimensions, any tracings of structures will be discarded (you will be given a opportunity to save them first). Nonetheless, it is always a good idea to save your traces before you import a new image.

If you have previously loaded or imported a file and choose to import a new first image volume with the same dimensions, you will be given the option to discard or to retain any existing traces.

File importing in MultiTracer relies on public domain Java classes in Wayne Rasband's ImageJ 1.29 program. If you want to help support importing of additional file formats into future versions of MultiTracer, please consider contributing code to Wayne Rasband for incorporation into ImageJ.

Modified: April 22, 2003